Temporal restriction of Cas9 expression improves CRISPR-mediated deletion efficacy and fidelity
Clinical application of CRISPR-Cas9 technology for large deletions of somatic mutations is inefficient, and methods to improve utility suffer from our inability to rapidly assess mono- vs.biallelic deletions.Here we Grooming kit establish a model system for investigating allelic heterogeneity at the single-cell level and identify indel scarring fro